The effect of intranasal administration of an IL-1b antagonist (RAIL) on the state of the nitroxydergic system of the brain during modeling of acute cerebrovascular accident

Introduction : This study was designed to evaluate the effect activity of RAIL-gel in comparison with Citicoline on nitroxydergic system during acute cerebrovascular accident. Methods In this study, 80 white nonlinear rats were randomly assigned 4 groups (20 each): 1) intact; 2) control - untreated accident (ACVA), examined th day; 3) animals ACVA, receiving RAIL, 18 4) treated Citicoline, day. The expression inducible NOS determined by Western blotting. nitrosative stress marker, nitrotyrosine, using ELISE kit NITROTYROSINE (kit no. HK501-02, series 12825k1212-k). To assess state iNOS mRNA expression, we used method polymerase chain reaction reverse transcription real time (RT-PCR). Results Our research demonstrated that course administration RAIL and ACVA for days leads stabilization parameters brain system. However, does not provide a full shifts NO brain. It have proper such an important link pathogenesis ischemic damage as stress. significant decrease due its form (decrease mRNA) metabolits, inhibition nitrotyrosine). Conclusion IL-1b antagonist (Intranal Gel) significantly exceeds terms severity indicators.